Detailed view for Tb927.6.1140

Basic information

TDR Targets ID: 18108
Trypanosoma brucei, dolichyl-P-Man:GDP-Man5GlcNAc2-PP-dolichyl alpha-1,2-mannosyltransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.8951 | Length (AA): 674 | MW (Da): 75203 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 10

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03901   Alg9-like mannosyltransferase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016757   transferase activity, transferring glycosyl groups  
GO:0031227   intrinsic to endoplasmic reticulum membrane  
GO:0006506   GPI anchor biosynthetic process  

Metabolic Pathways

N-Glycan biosynthesis (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
196 275 2onk (C) 50 125 32.00 0 0.05 0.0865944 2.58

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129144)

Species Accession Gene Product
Arabidopsis thaliana AT1G16900   Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
Brugia malayi Bm1_09555   Plasmid Maintenance Protein containing protein
Candida albicans CaO19.11922   similar to S. cerevisiae endoplasmic reticulum mannosyltranferase
Candida albicans CaO19.4442   similar to S. cerevisiae endoplasmic reticulum mannosyltranferase
Caenorhabditis elegans CELE_C14A4.3   Protein C14A4.3
Dictyostelium discoideum DDB_G0279349   dolichyl-phosphate-mannose alpha-1,2-mannosyltransferase
Drosophila melanogaster Dmel_CG11851   CG11851 gene product from transcript CG11851-RA
Echinococcus granulosus EgrG_000455200   glycosyltransferase
Echinococcus multilocularis EmuJ_000455200   glycosyltransferase
Homo sapiens ENSG00000086848   ALG9, alpha-1,2-mannosyltransferase
Leishmania donovani LdBPK_120145.1   dolichyl-P-Man:GDP-Man5GlcNAc2-PP-dolichyl alpha-1,2-mannosyltransferase, putative
Leishmania infantum LinJ.12.0140   Alg9-like mannosyltransferase, putative
Leishmania major LmjF.12.0160   dolichyl-P-Man:GDP-Man5GlcNAc2-PP-dolichyl alpha-1,2-mannosyltransferase, putative
Leishmania mexicana LmxM.12.0160   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_00676   plasmid Maintenance protein containing protein
Mus musculus ENSMUSG00000032059   asparagine-linked glycosylation 9 (alpha 1,2 mannosyltransferase)
Oryza sativa 4325533   Os01g0209000
Onchocerca volvulus OVOC10426   Putative glycosyltransferase
Saccharomyces cerevisiae YNL219C   dolichyl-P-Man:Man(6)GlcNAc(2)-PP-dolichol alpha-1,2-mannosyltransferase
Schistosoma japonicum Sjp_0106360   ko:K03846 alpha-1,2-mannosyltransferase, putative
Schistosoma mansoni Smp_103930.6   glycosyltransferase
Schistosoma mansoni Smp_103930.2   glycosyltransferase
Schistosoma mansoni Smp_103930.5   glycosyltransferase
Schmidtea mediterranea mk4.001456.05   Glycosyltransferase, putative
Trypanosoma brucei gambiense Tbg972.6.840   Alg9-like mannosyltransferase, putative
Trypanosoma brucei Tb927.6.1140   dolichyl-P-Man:GDP-Man5GlcNAc2-PP-dolichyl alpha-1,2-mannosyltransferase, putative
Trypanosoma cruzi TcCLB.509429.220   dolichyl-P-Man:GDP-Man5GlcNAc2-PP-dolichyl alpha-1,2-mannosyltransferase, putative
Trypanosoma cruzi TcCLB.507603.150   dolichyl-P-Man:GDP-Man5GlcNAc2-PP-dolichyl alpha-1,2-mannosyltransferase, putative

Essentiality

Tb927.6.1140 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.1140 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.1140 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.1140 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.1140 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.6.1140 (Trypanosoma brucei), dolichyl-P-Man:GDP-Man5GlcNAc2-PP-dolichyl alpha-1,2-mannosyltransferase, putative
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